Designing multi-input protein switches based on the conformational ensemble model of allostery
April 22, 2015
Jay and Abby's work on the semi-rational design of multi-input protein switches was published today in Nature Communications. The paper demosntrates how allostery can be established in a non-allosteric fusion protein (between maltose binding protein and TEM1 β-lactamase) through the introduction of mutations designed to increase the conformational entropy of the β-lactamase domain. Biophysical studies support our hypothesis. Our work adds to the growing appreciation of how intrinsically disordered regions can contribute to protein function and lends further support to the conformational ensemble model of allostery. The work was a collaboration with Vince Hilser (JHU).


Improved protein switches with therapeutic potential
November 26, 2014
Clay and Arjun's work on improving our cytosine deaminase switches for use in combination with prodrug therapy is published in PLoS One.

Biotechniques features our targeted methylation research
October 8, 2014
The September issue of Biotechniques has an article on targeting DNA methylation to the genome that features Brian's recent paper in PLoS One.


Jay Choi's paper on protein logic gates published
August 21, 2014
Today ACS Synthetic Biology published Jay Choi's paper on the rational design of protein switches to function as logic gates. The paper describes an alternative approach to gene circuits for introducing logic control elements into cells.


Another new NSF grant
June 17, 2014
More good news from NSF. The lab received a grant from the NSF-DEB Evolutionary Genetics Program to study epistasis.

Brian's paper on improved targeted methyltransferases published
May 8, 2014
Brian's paper on the creation of highly specific DNA methyltransrerases was published today in PLoS One.

New NSF grant on directed evolution
April 21, 2014
The lab received a new grant from the NSF-CBET Biotechnology, Biochemical, and Biomass Engineering Program to explore alternative pathways in directed evolution.

Tiana Warren offered two fellowships
April 18, 2014
Congratulations to Tiana Warren for being selected to receive both an NSF Predoctoral Fellowship and a Ford Foundation Fellowship. Tiana plans to accept the NSF Fellowship.

Marc Ostermeier elected to AIMBE
March 24, 2014
Marc Ostermeier was elected as a Fellow in the American Institute for Medical and Biological Engineering.

Dr. Clay Wright!
March 14, 2014
Congratulations to Clay Wright for successfully defending his thesis today. Next up for Clay (besides getting married) is a postdoc at The University of Washington with Jennifer Nemhauser in the Department of Biology.

Elad's fitness landscape paper published
February 23, 2014
Elad's paper describing a comprehensive map of the mutational landscape of the TEM-1 β-lactamase gene was published online today in Molecular Biology and Evolution.

Dr. Brian Chaikind!
January 17, 2014
Congratulations to Brian Chaikind for successfully defending his thesis today. Next up for Brian is a postdoc at Harvard with David Liu.


J. H. Choi, A. H. Laurent, V. J. Hilser, M. Ostermeier
Design of protein switches based on an ensemble model of allostery
Nat. Commun., 6:6968 (2015) [full text]

J. H. Choi, M. Ostermeier
Rational design of a fusion protein to exhibit disulfide-mediated logic gate behavior
ACS Synth. Biol., 4, 400-406 (2015) [full text]

L. S.-L. Cheung, D. J. Shea, N. Nicholes, A. Date, M. Ostermeier, K. Konstantopoulos
Characterization of monobody scaffold interactions with ligand via force spectroscopy and steered molecular dynamics
Sci. Rep., 5: 8247 (2015)

R. C. Wright, A. Khakhar, J. R. Eshleman, M. Ostermeier
Advancements in the development of HIF-1α-activated protein switches for use in enzyme prodrug therapy
PLoS One, 9(11): e114032 (2014) [full text]

J. H. Russell, M. Ostermeier
The thymidylate kinase genes from Mycobacterium tuberculosis and methicillin resistant Staphylococcus auerus confer 3'-azido-3'-deoxythymidine resistance to Escherichia coli
FEMS Microbiol. Lett., 361, 158-165 (2014)

B.Chaikind, M. Ostermeier
Directed evolution of improved zinc finger methyltransferases
PLoS One, 9(5): e96931 (2014) [full text]

E. Firnberg, J. W. Labonte, J. J. Gray, M. Ostermeier
A comprehensive, high-resolution map of a gene's fitness landscape
Mol. Bio. Evol., 31, 1581-1592 (2014) [PDF]

E. Firnberg, M. Ostermeier
The genetic code constrains yet facilitates Darwinian evolution
Nucleic Acids Res., 41, 7420-7428 (2013) [full text]

        Mutational tolerance in TEM-1 β-lactamase.